Engineering Physics Annotation << Back Decontamination of Surfaces and Biological Objects using a Modular Low-Temperature Plasma Source Based on a Barrier Discharge Konchekov E.M., Konkova A.S., Burmistrov D.E., Gudkova V.V., Knyazev A.V., Grudiev E.I., Stepanenko A.A., Kolik L.V., Gusein-Zade N.G. The use of low-temperature atmospheric pressure plasma for decontamination of surfaces of various nature, including biological objects, is of great interest in such areas of activity as medicine, agriculture and food industry. One of the most common methods of generating low-temperature plasma for solving this problem is the use of a barrier discharge, due to which it is possible to achieve a relatively large area of effective action and ensure the safety of heat-sensitive objects. The article studies the bactericidal activity of the plasma source based on a barrier discharge developed by us, and compares the results with other promising methods of generating low-temperature plasma: using a direct piezoelectric discharge and an argon plasma jet. Using a model of contaminated phosphate buffer, the contribution of active oxygen and nitrogen species to the antibacterial action was studied. Using a model of contaminated agar, the effective area of action was studied. The BR-based source demonstrates the largest area of action among the studied modes, while it is inferior in the effi ciency of producing active oxygen and nitrogen species in a phosphate buffer. Keywords: low-temperature plasma, barrier discharge, direct piezoelectric discharge, plasma jet, active oxygen forms, active nitrogen forms, bactericidal effect. DOI: 10.25791/infi zik.1.2025.1452 Pp. 18-27.