Engineering Physics Annotation << Back ANALYTICAL CAPACITY OF STIMULATED MID-IR EMISSION TO IN SITU SPECTRAL ANALYSIS OF PLANTS E.L. TERPUGOV, O.V. DEGTYAREVA, S.E. TERPUGOVA, V.V. SAVRANSKY In this paper, we present our study on the spectral analysis of fresh leaves using lightinduced mid-IR emission spectroscopy (LIMIRES), which is a technique that utilizes the CW optical pumping in order to prepare and monitor vibrtaionally-excited states of chromophores. We show that by using a low intense visible light and a sensitive FT-IR technique, it is possible to obtain high-quality IR-emission spectra of intact leaves. By varying illumination condition we were able to carry out the spectral discrimination between leaf samples of wild-type and transgenica tobacco plants. Results show that LIMIRES is a valuable new technique for studying the vibrational structure of excited pigments as well as the non destructive monitoring of tobacco leaf quality. Keywords: FT-IR, mid-infrared emission, optical pumping, multi-wave mixing, fresh tobacco leaves. Contacts: E-mail: el_terpugov@rambler.ru Pp. 51-58.